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Given the smaller sample size of children with Stage 2, we next combined the most severe stages (2 and 3) and assessed differences in GPx3 concentration, to increase statistical confidence

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All experimental procedures were performed in accordance with the requirements of the Animal Ethics Committee of the Tongji Medical College, Huazhong University of Science and Technology

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RNA was extracted with acid phenolchloroform (Klamar, 1227173473

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